Detection of Ergosterol Levels in Oil Palm Seedlings (Elaeis guineensis) Infected with Ganoderma Boninense in Hydroponic Media
Tutik Nurhidayati
Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Rizky Amalia Sandyaputri
Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Triono Bagus Saputro
Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Maya Shovitri
Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Nengah Dwianita Kuswytasari
Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Aulia Nasution
Department of Engineering Physics, Faculty of Industrial Technology and Systems Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Hendro Nurhadi
Department of Industrial Mechanical Engineering, Vocational Faculty, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Mashuri
Department of Industrial Mechanical Engineering, Vocational Faculty, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
ABSTRACT:
One of the diseases that attacks oil palm (Elaeis guineensis) is the Basal Stem Rot (BSR) disease caused by the Ganoderma boninense fungus. One method of early detection of BSR disease is ergosterol detection. Ergosterol is a sterol found in fungal cells. Therefore, ergosterol can be used as a biomarker. The aim of this research was to detect ergosterol levels in oil palm seedlings infected with the fungus G. boninense which were cultivated in hydroponic media. The method used in the study was oil palm cultivation in hydroponic media. Oil palm plants that were 45 days old after planting in hydroponic media were then infected with G. boninense fungus. The inoculum source used RWB (Rubber Wood Block) which had been infected with G. boninense fungus. Furthermore, plant harvesting was carried out every day for 12 times. The research design used was a Completely Randomized Design (CRD) consisting of 2 treatments, namely control and treatment of G. boninense inoculum administration on hydroponic media. Each treatment was repeated 3 times. Observations were carried out in series, namely on the third day, sixth day, ninth day, and twelfth day. The observation samples to be taken were the roots, stems, and leaves. Furthermore, the harvest results were detected for ergosterol content using the UV-Vis Spectrophotometry method. The results showed that ergosterol levels in roots, stems, and leaves of oil palm seedlings (E. guineensis) infected by G. boninense fungus in hydroponic media increased significantly along with the observation days. The highest ergosterol levels were in the root organ: 53.79 mg/L; stem organ: 86.38 mg/L and in the leaf organ: 75.52 mg/L.
Published in: International Journal of Research in Engineering, Science and Management (Volume 7, Issue 11, November 2024)
Page(s): 22-24
Date of Publication: 14/11/2024
Publisher: IJRESM
Cite as: Tutik Nurhidayati, Rizky Amalia Sandyaputri, Triono Bagus Saputro, Maya Shovitri, Nengah Dwianita Kuswytasari, Aulia Nasution, Hendro Nurhadi, Mashuri, “Detection of Ergosterol Levels in Oil Palm Seedlings (Elaeis guineensis) Infected with Ganoderma Boninense in Hydroponic Media,” in International Journal of Research in Engineering, Science and Management, vol. 7, no. 11, pp. 22-24, November 2024.